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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR)

    WB analysis using 84739-1-RR

    Non-treated HeLa cels, NIH/3T3 cells, HSC-T6 cells, and Calyculin A treated HeLa cels, NIH/3T3 cells, HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84739-1-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR)

    IF Staining of HeLa using 84739-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84739-1-RR, Clone: 241897G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR)

    FC experiment of HeLa using 84739-1-RR

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR, Clone:241897G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Phospho-Histone H3 (Thr3) Recombinant antibody

Phospho-Histone H3 (Thr3) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84739-1-RR
Clone No.241897G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Histone H3 (Thr3), p Histone H3, p Histone H3 (Thr3), p Histone H3 Thr3, p-Histone H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HSC-T6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84739-1-RR targets Phospho-Histone H3 (Thr3) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672123
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.

Protocols

Product Specific Protocols
WB protocol for Phospho-Histone H3 (Thr3) antibody 84739-1-RRDownload protocol
IF protocol for Phospho-Histone H3 (Thr3) antibody 84739-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols