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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR)

    WB analysis using 84739-1-RR (same clone as 84739-1-PBS)

    Non-treated HeLa cels, NIH/3T3 cells, HSC-T6 cells, and Calyculin A treated HeLa cels, NIH/3T3 cells, HSC-T6 cells were subjected to SDS PAGE followed by western blot with 84739-1-RR (Phospho-Histone H3 (Thr3) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR)

    IF Staining of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84739-1-RR, Clone: 241897G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR)

    FC experiment of HeLa using 84739-1-RR (same clone as 84739-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.06 ug Phospho-Histone H3 (Thr3) Recombinant antibody (84739-1-RR, Clone:241897G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84739-1-PBS in a different storage buffer formulation.

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Phospho-Histone H3 (Thr3) Recombinant antibody, PBS Only

Phospho-Histone H3 (Thr3) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84739-1-PBS
Clone No.241897G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HIST1H3A, 241897G3, Histone-H3, HistoneH3, Histone H3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84739-1-PBS targets Phospho-Histone H3 (Thr3) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.