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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 647-conjugated Phospho-HER2/ErbB2 ( (CL647-82762-11)

    FC experiment of A431 using CL647-82762-11

    1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Her2/Erbb2 (Tyr877) Recombinant Antibody (CL647-82762-11, Clone:250539F5)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

CoraLite® Plus 647-conjugated Phospho-HER2/ErbB2 (Tyr877) Recombinant monoclonal antibody

Phospho-HER2/ErbB2 (Tyr877) Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. CL647-82762-11
Clone No.250539F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

ErbB2 (Tyr877), HER2 (Tyr877), HER2/ErbB2 (Tyr877), P-HER2/ErbB2, P-HER2/ErbB2 (Tyr877)

Formulation:  PBS, Proclin300, BSA, Glycerol
PBS, Proclin300, BSA, Glycerol
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inEGF treated A431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-82762-11 targets Phospho-HER2/ErbB2 (Tyr877) in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated molecular weight 1255 aa, 138 kDa
Observed molecular weight185 kDa
GenBank accession numberBC156755
Gene Symbol HER2/ErbB2
Gene ID (NCBI) 2064
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
激发激光Red Laser (633 nm)
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04626
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301)

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 Phospho-HER2/ErbB2 (Tyr877) antibody CL647-82762-11Download protocol
Standard Protocols
Click here to view our Standard Protocols
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