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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Phospho-Filamin A (Ser2152) Recombinant monoclonal (87513-1-RR)

    WB analysis of A431 using 87513-1-RR

    Non-treated A431 cells and EGF treated A431 cells were subjected to SDS PAGE followed by western blot with 87513-1-RR (Phospho-Filamin A (Ser2152) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

Phospho-Filamin A (Ser2152) Recombinant monoclonal antibody

Phospho-Filamin A (Ser2152) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87513-1-RR
Clone No.252855H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

p-Filamin A (S2152), p-Filamin A Ser2152, p-FLNA Ser2152, Phospho Filamin A (Ser2152), Phospho FLNA (Ser2152)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inEGF treated A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87513-1-RR targets Phospho-Filamin A (Ser2152) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name filamin A, alpha (actin binding protein 280)
Calculated molecular weight 2647 aa, 280 kDa
Observed molecular weight280 kDa
GenBank accession numberNM_001110556
Gene Symbol FLNA
Gene ID (NCBI) 2316
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP21333
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network. Phospho-Filamin A (Ser2152) is a phosphorylated form of Filamin A (FLNA), which is a large actin-binding protein that plays a crucial role in organizing the cytoskeleton and transducing signals from cell membrane to the cytoskeleton. This phosphorylation event is associated with various cellular functions, including cytoskeletal remodeling, cell migration, and focal adhesion formation. (PMID:29931263)

Protocols

Product Specific Protocols
WB protocol for Phospho-Filamin A (Ser2152) antibody 87513-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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