Validation Data Gallery
Tested Applications
| Positive WB detected in | EGF treated A431 cells |
Ezrin phosphorylates at Tyr354, sometimes called Tyr353.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88246-2-RR targets Phospho-Ezrin (Tyr354) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ezrin |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | NM_001111077 |
| Gene Symbol | Ezrin |
| Gene ID (NCBI) | 7430 |
| ENSEMBL Gene ID | ENSG00000092820 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15311 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. Phospho-Ezrin (Tyr354), also annotated as Tyr353 in early literature, is a tyrosine-phosphorylated active form of ERM scaffold protein Ezrin encoded by the EZR gene. Triggered by HGF receptor tyrosine kinase, this modification unlocks Ezrin's autoinhibited conformation and enables high-affinity binding to the p85 regulatory subunit of PI3K. It sustains PI3K/Akt survival signaling, suppresses epithelial apoptosis, and drives cytoskeleton remodeling for cell migration and tubulogenesis. Unique from Thr567-phosphorylated Ezrin at cell membranes, p-Ezrin (Tyr354) translocates into nuclei in osteosarcoma and lung cancer. Its elevated nuclear expression correlates with tumor invasion, metastasis, and poor clinical prognosis, marking it a promising malignant progression biomarker. (PMID: 10377409, 20348881)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Ezrin (Tyr354) antibody 88246-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |