Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
88246-2-PBS targets Phospho-Ezrin (Tyr354) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ezrin |
| Calculated molecular weight | 69 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | NM_001111077 |
| Gene Symbol | Ezrin |
| Gene ID (NCBI) | 7430 |
| ENSEMBL Gene ID | ENSG00000092820 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15311 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. Phospho-Ezrin (Tyr354), also annotated as Tyr353 in early literature, is a tyrosine-phosphorylated active form of ERM scaffold protein Ezrin encoded by the EZR gene. Triggered by HGF receptor tyrosine kinase, this modification unlocks Ezrin's autoinhibited conformation and enables high-affinity binding to the p85 regulatory subunit of PI3K. It sustains PI3K/Akt survival signaling, suppresses epithelial apoptosis, and drives cytoskeleton remodeling for cell migration and tubulogenesis. Unique from Thr567-phosphorylated Ezrin at cell membranes, p-Ezrin (Tyr354) translocates into nuclei in osteosarcoma and lung cancer. Its elevated nuclear expression correlates with tumor invasion, metastasis, and poor clinical prognosis, marking it a promising malignant progression biomarker. (PMID: 10377409, 20348881)