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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Estrogen Receptor α (Ser118) Recombinant a (84564-6-RR)

    WB analysis using 84564-6-RR

    T-47D cell lysates were subjected to SDS PAGE followed by western blot with 84564-6-RR (Phospho-Estrogen Receptor α (Ser118) antibody) blocked with BSA only, Phospho-Estrogen Receptor α (Ser118) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of MCF-7 cells using Phospho-Estrogen Receptor α (Ser118) Recombinant m (84564-6-RR)

    FC experiment of MCF-7 using 84564-6-RR

    1X10^6 MCF-7 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody (84564-6-RR, Clone:250278F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody

Phospho-Estrogen Receptor α (Ser118) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84564-6-RR
Clone No.250278F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Estrogen Receptor α (Ser118), p Estrogen Receptor α, p-ERS1, p-ERS1 (Ser118), p-Estrogen Receptor α

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inT-47D cells
Positive FC (Intra) detected inλ phosphatase treated MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84564-6-RR targets Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name estrogen receptor 1
Calculated molecular weight 595 aa, 66 kDa
Observed molecular weight66 kDa
GenBank accession numberBC128573
Gene Symbol ER
Gene ID (NCBI) 2099
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP03372
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)

Protocols

Product Specific Protocols
FC protocol for Phospho-Estrogen Receptor α (Ser118) antibody 84564-6-RRDownload protocol
WB protocol for Phospho-Estrogen Receptor α (Ser118) antibody 84564-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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