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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Cofilin (Ser3) Recombinant monoclonal anti (86307-1-RR)

    WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Phospho-Cofilin (Ser3) Recombinant monoclonal anti (86307-1-RR)

    WB analysis using 86307-1-RR (same clone as 86307-1-PBS)

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Hela cells using Phospho-Cofilin (Ser3) Recombinant monoclonal anti (86307-1-RR)

    IF Staining of Hela using 86307-1-RR (same clone as 86307-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-Cofilin (Ser3) antibody (86307-1-RR, Clone: 250986H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86307-1-PBS in a different storage buffer formulation.

Phospho-Cofilin (Ser3) Recombinant monoclonal antibody, PBS Only

Phospho-Cofilin (Ser3) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86307-1-PBS
Clone No.250986H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Cofilin (phospho S3), Cofilin (Ser3), p Cofilin, p-Cofilin, p-Cofilin (Ser3)

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86307-1-PBS targets Phospho-Cofilin (Ser3) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23528
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

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