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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-Cofilin (Ser3) Recombinant antibody (86307-1-RR)

    WB analysis using 86307-1-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-Cofilin (Ser3) Recombinant antibody (86307-1-RR)

    WB analysis using 86307-1-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86307-1-RR (Phospho-Cofilin (Ser3) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of Hela cells using Phospho-Cofilin (Ser3) Recombinant monoclonal anti (86307-1-RR)

    IF Staining of Hela using 86307-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated Hela cells using Phospho-Cofilin (Ser3) antibody (86307-1-RR, Clone: 250986H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-Cofilin (Ser3) Recombinant monoclonal antibody

Phospho-Cofilin (Ser3) Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86307-1-RR
Clone No.250986H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Cofilin (phospho S3), Cofilin (Ser3), p Cofilin, p-Cofilin, p-Cofilin (Ser3)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, λ phosphatase treated HeLa cells, λ phosphatase treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86307-1-RR targets Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cofilin 1 (non-muscle)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23528
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Protocols

Product Specific Protocols
IF protocol for Phospho-Cofilin (Ser3) antibody 86307-1-RRDownload protocol
WB protocol for Phospho-Cofilin (Ser3) antibody 86307-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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