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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-CBX3 (Ser93) Recombinant monoclonal antibo (87869-3-RR)

    WB analysis using 87869-3-RR

    Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-CBX3 (Ser93) Recombinant monoclonal antibo (87869-3-RR)

    WB analysis using 87869-3-RR

    Non-treated NIH/3T3 cells and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-CBX3 (Ser93) Recombinant monoclonal antibo (87869-3-RR)

    FC experiment of HeLa using 87869-3-RR

    1x10^6 HeLa cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-CBX3 (Ser93) Recombinant monoclonal antibody (87869-3-RR, Clone:260091A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-CBX3 (Ser93) Recombinant monoclonal antibody

Phospho-CBX3 (Ser93) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 87869-3-RR
Clone No.260091A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

CBX3 (Ser93), HP1 gamma (Ser83), p-CBX3, p-CBX3 (Ser93), Phospho-CBX3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inForskolin treated NIH/3T3 cells, paclitaxel treated HeLa cells
Positive FC (Intra) detected inpaclitaxel treated HeLa cells

This antibody, also known as HP1 gamma Ser83.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87869-3-RR targets Phospho-CBX3 (Ser93) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Calculated molecular weight 21 kDa
Observed molecular weight22 kDa
GenBank accession numberNM_016587
Gene Symbol CBX3
Gene ID (NCBI) 11335
RRIDAB_3745797
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13185
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CBX3, also known as HP1γ (Heterochromatin Protein 1 gamma), is a protein-coding gene located on human chromosome 7p15.2. Phospho-CBX3 (Ser93) refers to the phosphorylated form of Chromobox Homolog 3 (CBX3), also known as HP1γ (Heterochromatin Protein 1 gamma), at serine residue 93. This phosphorylation site is located in the N-terminal tail region of the protein and plays a regulatory role in chromatin binding and transcriptional control. (PMID: 39907700)

Protocols

Product Specific Protocols
FC protocol for Phospho-CBX3 (Ser93) antibody 87869-3-RRDownload protocol
WB protocol for Phospho-CBX3 (Ser93) antibody 87869-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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