CoraLite® Plus 647-conjugated Phospho-Beta Catenin (Ser675) Recombinant antibody
Phospho-Beta Catenin (Ser675) Recombinant Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
6D6
Cat no : CL647-80084
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | λ phosphatase treated MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL647-80084 targets Phospho-Beta Catenin (Ser675) in IF applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated molecular weight | 781 aa, 86 kDa |
| Observed molecular weight | 90-100 kDa |
| GenBank accession number | BC058926 |
| Gene symbol | CTNNB1 |
| Gene ID (NCBI) | 1499 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 Phospho-Beta Catenin (Ser675) antibody CL647-80084 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
