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Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
85703-1-PBS targets Phospho-ATG4B (Ser383) in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ATG4 autophagy related 4 homolog B (S. cerevisiae) |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 44-48 kDa |
| GenBank accession number | BC000719 |
| Gene Symbol | ATG4B |
| Gene ID (NCBI) | 23192 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y4P1 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)