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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-ATG4B (Ser383) Recombinant antibody (85703-1-RR)

    WB analysis using 85703-1-RR (same clone as 85703-1-PBS)

    Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 85703-1-RR (Phospho-ATG4B (Ser383) antibody) blocked with BSA only, Phospho-ATG4B (Ser383) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85703-1-PBS in a different storage buffer formulation.

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Phospho-ATG4B (Ser383) Recombinant antibody, PBS Only

Phospho-ATG4B (Ser383) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85703-1-PBS
Clone No.242927A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

ATG4B, APG4B, AUTL1, AUT-like 1 cysteine endopeptidase, Autophagin-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85703-1-PBS targets Phospho-ATG4B (Ser383) in applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ATG4 autophagy related 4 homolog B (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight44-48 kDa
GenBank accession numberBC000719
Gene Symbol ATG4B
Gene ID (NCBI) 23192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4P1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATG4B stimulates autophagy by promoting autophagosome formation through reversible modification of ATG8. In humans, microtubule-associated protein 1 light chain, LC3B, is the best-characterized ATG8 isoform, and ATG4B, but not three other ATG4 isoforms, displays a highly selective preference toward LC3B. MST4 phosphorylates ATG4B at serine residue 383, which stimulates ATG4B activity and increases autophagic flux. (PMID: 29232556, PMID: 36056541)