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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-AKT (Ser473) Recombinant monoclonal antibo (80462-2-RR)

    WB analysis using 80462-2-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80462-2-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (60004-1-Ig) as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-AKT (Ser473) Recombinant monoclonal antibo (80462-2-RR)

    WB analysis using 80462-2-RR

    Non-treated and Calyculin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80462-2-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (60004-1-Ig) as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-AKT (Ser473) Recombinant monoclonal antibo (80462-2-RR)

    WB analysis using 80462-2-RR

    Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80462-2-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (60004-1-Ig) as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-AKT (Ser473) Recombinant monoclonal antibo (80462-2-RR)

    WB analysis using 80462-2-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80462-2-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (HRP-81640-5) as loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-AKT (Ser473) Recombinant monoclonal antibo (80462-2-RR)

    WB analysis using 80462-2-RR

    Non-treated and Calyculin A treated C2C12 cells were subjected to SDS PAGE followed by western blot with 80462-2-RR (Phospho-AKT (Ser473) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (HRP-81640-5) as loading control.

Phospho-AKT (Ser473) Recombinant monoclonal antibody

Phospho-AKT (Ser473) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 80462-2-RR
Clone No.253264G12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

P AKT (Ser473), P-AKT (Ser473), Phos AKT (Ser473), Phos-AKT (Ser473), Phospho AKT (Ser473)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCalyculin A treated HepG2 cells, Calyculin A treated C2C12 cells, HEK-293 cells, Calyculin A treated HSC-T6 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80462-2-RR targets Phospho-AKT (Ser473) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 1
Observed molecular weight 58 kDa
GenBank accession numberNM_005163
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)

Protocols

Product Specific Protocols
WB protocol for Phospho-AKT (Ser473) antibody 80462-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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