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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR)

    WB analysis using 86599-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86599-1-RR (Peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR)

    IF Staining of HepG2 using 86599-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Peroxiredoxin 2 antibody (86599-1-RR, Clone: 251494D3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of C2C12 cells using Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR)

    FC experiment of C2C12 using 86599-1-RR

    1x10^6 C2C12 cells were intracellularly stained with 0.25 ug Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR, Clone:251494D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Affinity and Kinetic Characterization of 86599-1-RR

    Affinity and Kinetic Characterization of 86599-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86599-1-RR against Human Peroxiredoxin 2 were performed. The affinity constant is 3.01 nM.

Peroxiredoxin 2 Recombinant monoclonal antibody

Peroxiredoxin 2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 86599-1-RR
Clone No.251494D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PRDX2, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB, NKEF-B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-12 cells, C2C12 cells, mouse brain tissue, rat brain tissue, rat spleen tissue, rat heart tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inC2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86599-1-RR targets Peroxiredoxin 2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0835

Product name: Recombinant human PRDX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC003022

Sequence: MASGNARIGKPAPDFKATAVVDGAFKEVKLSDYKGKYVVLFFYPLDFTFVCPTEIIAFSNRAEDFRKLGCEVLGVSVDSQFTHLAWINTPRKEGGLGPLNIPLLADVTRRLSEDYGVLKTDEGIAYRGLFIIDGKGVLRQITVNDLPVGRSVDEALRLVQAFQYTDEHGEVCPAGWKPGSDTIKPNVDDSKEYFSKHN

相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC003022
Gene Symbol peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_3744995
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP32119
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxiredoxin 2 (PRDX2), also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Protocols

Product Specific Protocols
FC protocol for Peroxiredoxin 2 antibody 86599-1-RRDownload protocol
IF protocol for Peroxiredoxin 2 antibody 86599-1-RRDownload protocol
WB protocol for Peroxiredoxin 2 antibody 86599-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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