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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR)

    WB analysis using 86599-1-RR (same clone as 86599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86599-1-RR (Peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86599-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02542-2

    Sandwich ELISA sample test of MP02542-2

    The mean Peroxiredoxin 2 concentration was determined to be 75.74 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP02542-2

    Sandwich ELISA sample test of MP02542-2

    Plasma of six individual healthy human donors was measured. The human Peroxiredoxin 2 concentration of detected samples was determined to be 6.52 ng/mL with a range of 5.21 - 7.71 ng/mL.

  • Sandwich ELISA standard curve of MP02542-2

    Sandwich ELISA standard curve of MP02542-2

    Sandwich ELISA standard curve of MP02542-2, Human Peroxiredoxin 2 Recombinant Matched Antibody Pair - PBS only. 86599-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0835. 86599-1-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR)

    IF Staining of HepG2 using 86599-1-RR (same clone as 86599-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Peroxiredoxin 2 antibody (86599-1-RR, Clone: 251494D3 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86599-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of C2C12 cells using Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR)

    FC experiment of C2C12 using 86599-1-RR (same clone as 86599-1-PBS)

    1x10^6 C2C12 cells were intracellularly stained with 0.25 ug Peroxiredoxin 2 Recombinant monoclonal antibody (86599-1-RR, Clone:251494D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 86599-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02542-1

    Cytometric bead array sample test of MP02542-1

    The mean Peroxiredoxin 2 concentration was determined to be 144.8 ng/mL in human serum.

  • Cytometric bead array sample test of MP02542-1

    Cytometric bead array sample test of MP02542-1

    The mean Peroxiredoxin 2 concentration was determined to be 170.3 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02542-1

    Cytometric bead array standard curve of MP02542-1

    Cytometric bead array standard curve of MP02542-1, Peroxiredoxin 2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86599-2-PBS. Detection antibody: 86599-1-PBS. Standard: Ag0835. Range: 3.125-100 ng/mL.

  • Affinity and Kinetic Characterization of 86599-1-RR

    Affinity and Kinetic Characterization of 86599-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86599-1-RR against Human Peroxiredoxin 2 were performed. The affinity constant is 3.01 nM.

Peroxiredoxin 2 Recombinant monoclonal antibody, PBS Only (Detector)

Peroxiredoxin 2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 86599-1-PBS
Clone No.251494D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

PRDX2, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB, NKEF-B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86599-1-PBS targets Peroxiredoxin 2 as part of a matched antibody pair:

MP02542-1: 86599-2-PBS capture and 86599-1-PBS detection (validated in Cytometric bead array)

MP02542-2: 86599-3-PBS capture and 86599-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0835

Product name: Recombinant human PRDX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC003022

Sequence: MASGNARIGKPAPDFKATAVVDGAFKEVKLSDYKGKYVVLFFYPLDFTFVCPTEIIAFSNRAEDFRKLGCEVLGVSVDSQFTHLAWINTPRKEGGLGPLNIPLLADVTRRLSEDYGVLKTDEGIAYRGLFIIDGKGVLRQITVNDLPVGRSVDEALRLVQAFQYTDEHGEVCPAGWKPGSDTIKPNVDDSKEYFSKHN

相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC003022
Gene Symbol peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_3744995
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP32119
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Peroxiredoxin 2 (PRDX2), also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

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