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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Parvalbumin Recombinant antibody (85819-4-RR)

    IHC staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85819-4-RR (PVALB antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Parvalbumin Recombinant antibody (85819-4-RR)

    IHC staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85819-4-RR (PVALB antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Parvalbumin Recombinant antibody (85819-4-RR)

    IF Staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (85819-4-RR, Clone: 250158B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Parvalbumin Recombinant antibody (85819-4-RR)

    IF Staining of mouse cerebellum using 85819-4-RR (same clone as 85819-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (85819-4-RR, Clone: 250158B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85819-4-PBS in a different storage buffer formulation.

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Parvalbumin Recombinant antibody, PBS Only

Parvalbumin Uni-rAbTM Recombinant Antibody for IHC, IF-P, Indirect ELISA
Cat No. 85819-4-PBS
Clone No.250158B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

PVALB, Parvalbumin alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85819-4-PBS targets Parvalbumin in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Parvalbumin fusion protein Ag30189 相同性解析による交差性が予測される生物種
Full Name parvalbumin
Calculated molecular weight 12 kDa
GenBank accession numberNM_002854
Gene Symbol Parvalbumin
Gene ID (NCBI) 5816
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20472
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).