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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Parvalbumin Recombinant antibody (85819-4-RR)

    IHC staining of mouse cerebellum using 85819-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85819-4-RR (PVALB antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Parvalbumin Recombinant antibody (85819-4-RR)

    IHC staining of mouse cerebellum using 85819-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85819-4-RR (PVALB antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Parvalbumin Recombinant monoclonal antibody (85819-4-RR)

    IF Staining of mouse cerebellum using 85819-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (85819-4-RR, Clone: 250158B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using Parvalbumin Recombinant monoclonal antibody (85819-4-RR)

    IF Staining of mouse cerebellum using 85819-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Parvalbumin antibody (85819-4-RR, Clone: 250158B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85819-4-RR

    Affinity and Kinetic Characterization of 85819-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85819-4-RR against Human Parvalbumin were performed. The affinity constant is 0.715 nM.

Parvalbumin Recombinant monoclonal antibody

Parvalbumin Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 85819-4-RR
Clone No.250158B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

PVALB, Parvalbumin alpha

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85819-4-RR targets Parvalbumin in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag30189

Product name: Recombinant human PVALB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-110 aa of NM_002854

Sequence: SMTDLLNAEDIKKAVGAFSATDSFDHKKFFQMVGLKKKSADDVKKVFHMLDKDKSGFIEEDELGFILKGFSPDARDLSAKETKMLMAAGDKDGDGKIGVDEFSTLVAES

相同性解析による交差性が予測される生物種
Full Name parvalbumin
Calculated molecular weight 12 kDa
GenBank accession numberNM_002854
Gene Symbol Parvalbumin
Gene ID (NCBI) 5816
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20472
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PVALB is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. PVALB is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. It is thought to be involved in muscle relaxation. Monomeric (12 kDa) and dimeric (24 kDa) forms, as well as trimeric Parvalbumin (38 kDa), have been described in a literature (PMID: 19616851).

Protocols

Product Specific Protocols
IF protocol for Parvalbumin antibody 85819-4-RRDownload protocol
IHC protocol for Parvalbumin antibody 85819-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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