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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using PE-conjugated Pan-Keratin (TypeⅡ) Recombinant mono (PE-86370)

    IF Staining of human colon cancer using PE-86370

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using PE-conjugated Pan-Keratin (TypeⅡ) Recombinant mono (PE-86370)

    IF Staining of human colon cancer using PE-86370

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using PE-conjugated Pan-Keratin (TypeⅡ) Recombinant mono (PE-86370)

    IF Staining of human cervical cancer using PE-86370

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human cervical cancer tissue using PE-conjugated Pan-Keratin (TypeⅡ) Recombinant mono (PE-86370)

    IF Staining of human cervical cancer using PE-86370

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PE-conjugated Pan-Keratin (TypeⅡ) Recombinant mono (PE-86370)

    IF Staining of human lung cancer using PE-86370

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using PE-conjugated Pan-Keratin (TypeⅡ) Recombinant mono (PE-86370)

    IF Staining of human lung cancer using PE-86370

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PE-conjugated Pan-Keratin (TypeⅡ) Recombinant monoclonal antibody

Pan-Keratin (TypeⅡ) Uni-rAb® Recombinant Antibody for IF-P
Cat No. PE-86370
Clone No.251040F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

Pan Keratin, Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins

Formulation:  PBS, Azide, BSA
PBS, Azide, BSA
Conjugate:  PE
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman colon cancer tissue, human lung cancer tissue, human cervical cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-86370 targets Pan-Keratin (TypeⅡ) in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name Pan-Keratin (TypeⅡ)
Observed molecular weight52-68 kDa
Gene Symbol
Gene ID (NCBI)
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14533
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86. 86370-1-RR can recognize KRT81/82/83/85/86.

Protocols

Product Specific Protocols
IF protocol for PE Pan-Keratin (TypeⅡ) antibody PE-86370Download protocol
Standard Protocols
Click here to view our Standard Protocols
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