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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTH1R Recombinant monoclonal antibody (87076-3-RR)

    WB analysis using 87076-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87076-3-RR (PTH1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PTH1R Recombinant monoclonal antibody (87076-3-RR)

    WB analysis of HEK-293 using 87076-3-RR

    WB result of PTH1R antibody (87076-3-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTH1R transfected HEK-293 cells.

  • Western Blot (WB) analysis of mouse kidney tissue using PTH1R Recombinant monoclonal antibody (87076-3-RR)

    WB analysis of mouse kidney using 87076-3-RR

    Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 87076-3-RR (PTH1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • KD/KO Validated

PTH1R Recombinant monoclonal antibody

PTH1R Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87076-3-RR
Clone No.252173F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Parathyroid hormone/parathyroid hormone-related peptide receptor, PFE, PTHR, PTHR1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse kidney tissue, HepG2 cells, MCF-7 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87076-3-RR targets PTH1R in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6501

Product name: Recombinant Human PTH1R protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-188 aa of NM_000316.2

Sequence: DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLG

相同性解析による交差性が予測される生物種
Full Name parathyroid hormone 1 receptor
Calculated molecular weight66 kDa
Observed molecular weight66 kDa and 90 kDa
GenBank accession numberNM_000316.2
Gene Symbol PTH1R
Gene ID (NCBI) 5745
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ03431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. The calculated molecular weight of PTH1R is 66 kDa, and PTH1R can form a 120 kDa dimer in the non-reducing condition. With glycosylation modification, the molecular weight of PTH1R will migrate to 90 kDa (PMID: 16783882).

Protocols

Product Specific Protocols
WB protocol for PTH1R antibody 87076-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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