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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTH1R Recombinant monoclonal antibody (87076-3-RR)

    WB analysis using 87076-3-RR (same clone as 87076-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87076-3-RR (PTH1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87076-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PTH1R Recombinant monoclonal antibody (87076-3-RR)

    WB analysis of HEK-293 using 87076-3-RR (same clone as 87076-3-PBS)

    WB result of PTH1R antibody (87076-3-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTH1R transfected HEK-293 cells. This data was developed using the same antibody clone with 87076-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using PTH1R Recombinant monoclonal antibody (87076-3-RR)

    WB analysis of mouse kidney using 87076-3-RR (same clone as 87076-3-PBS)

    Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 87076-3-RR (PTH1R antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87076-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02999-1

    Sandwich ELISA sample test of MP02999-1

    The mean PTH1R concentration was determined to be 63.56 ng/mL in 786-O cell extract based on a 1.20 mg/mL extract load and 57.87 ng/mL in HK-2 cell extract based on a 1.20 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02999-1

    Sandwich ELISA standard curve of MP02999-1

    Sandwich ELISA standard curve of MP02999-1, Human PTH1R Recombinant Matched Antibody Pair - PBS only. 87076-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6501. 87076-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

PTH1R Recombinant monoclonal antibody, PBS Only (Capture)

PTH1R Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 87076-3-PBS
Clone No.252173F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

Parathyroid hormone/parathyroid hormone-related peptide receptor, PFE, PTHR, PTHR1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87076-3-PBS targets PTH1R as part of a matched antibody pair:

MP02999-1: 87076-3-PBS capture and 87076-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg6501

Product name: Recombinant Human PTH1R protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-188 aa of NM_000316.2

Sequence: DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLG

相同性解析による交差性が予測される生物種
Full Name parathyroid hormone 1 receptor
Calculated molecular weight66 kDa
Observed molecular weight66 kDa and 90 kDa
GenBank accession numberNM_000316.2
Gene Symbol PTH1R
Gene ID (NCBI) 5745
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ03431
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. The calculated molecular weight of PTH1R is 66 kDa, and PTH1R can form a 120 kDa dimer in the non-reducing condition. With glycosylation modification, the molecular weight of PTH1R will migrate to 90 kDa (PMID: 16783882).

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