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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMG4 Polyclonal antibody (31201-1-AP)

    WB analysis using 31201-1-AP (same clone as 31201-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31201-1-AP (PSMG4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31201-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PSMG4 Polyclonal antibody (31201-1-AP)

    IF Staining of A431 using 31201-1-AP (same clone as 31201-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PSMG4 antibody (31201-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31201-1-PBS in a different storage buffer formulation.

PSMG4 Polyclonal antibody, PBS Only

PSMG4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31201-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

bA506K6.2, C6orf86, hPAC4, PAC 4, PAC4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31201-1-PBS targets PSMG4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35222

Product name: Recombinant human PSMG4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-123 aa of NM_001128591.1

Sequence: MEGLVVAAGGDVSLHNFSARLWEQLVHFHVMRLTDSLFLWVGATPHLRNLAVAMCSRYDSIPVSTSLLGDTSDTTSTGLAQRLARKTNKQVFVSYNLQNTDSNFALLVENRIKEEMEAFPEKF

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) assembly chaperone 4
Calculated molecular weight14 kDa
Observed molecular weight13 kDa
GenBank accession numberNM_001128591.1
Gene Symbol PSMG4
Gene ID (NCBI) 389362
RRIDAB_3669898
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5JS54
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PSMG4 (Proteasome assembly chaperone 4), also known as C6orf86. It is expected to be located in the nucleus and cytoplasm, which interacts with PSMG3 and asociates with alpha subunits of the 20S proteasome. The molecular weight of PSMG4 is 13 kDa.

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