Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, MDA-MB-231 cells, SK-BR-3 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31201-1-AP targets PSMG4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35222 Product name: Recombinant human PSMG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of NM_001128591.1 Sequence: MEGLVVAAGGDVSLHNFSARLWEQLVHFHVMRLTDSLFLWVGATPHLRNLAVAMCSRYDSIPVSTSLLGDTSDTTSTGLAQRLARKTNKQVFVSYNLQNTDSNFALLVENRIKEEMEAFPEKF 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) assembly chaperone 4 |
| Calculated molecular weight | 14 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | NM_001128591.1 |
| Gene Symbol | PSMG4 |
| Gene ID (NCBI) | 389362 |
| RRID | AB_3669898 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q5JS54 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMG4 (Proteasome assembly chaperone 4), also known as C6orf86. It is expected to be located in the nucleus and cytoplasm, which interacts with PSMG3 and asociates with alpha subunits of the 20S proteasome. The molecular weight of PSMG4 is 13 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMG4 antibody 31201-1-AP | Download protocol |
| WB protocol for PSMG4 antibody 31201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |