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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using PSME1 Polyclonal antibody (10543-1-AP)

    WB analysis of mouse spleen using 10543-1-AP (same clone as 10543-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PSME1 Polyclonal antibody (10543-1-AP)

    WB analysis of A431 using 10543-1-AP (same clone as 10543-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using PSME1 Polyclonal antibody (10543-1-AP)

    WB analysis of RAW 264.7 using 10543-1-AP (same clone as 10543-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSME1 Polyclonal antibody (10543-1-AP)

    IHC staining of human lung cancer using 10543-1-AP (same clone as 10543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PSME1 Polyclonal antibody (10543-1-AP)

    IHC staining of human lung cancer using 10543-1-AP (same clone as 10543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10543-1-PBS in a different storage buffer formulation.

PSME1 Polyclonal antibody, PBS Only

PSME1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10543-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10543-1-PBS targets PSME1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0831

Product name: Recombinant human PSME1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-249 aa of BC007503

Sequence: MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Calculated molecular weight 29 kDa
Observed molecular weight 29-33 kDa
GenBank accession numberBC007503
Gene Symbol PSME1
Gene ID (NCBI) 5720
RRIDAB_2170944
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06323
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

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