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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSME1 Polyclonal antibody

PSME1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10543-1-AP

Synonyms

IFI5111, IGUP I 5111, PA28A, PA28alpha, PSME1, REG alpha, REGalpha

Validation Data Gallery

Applications:
  • WB analysis of mouse spleen using 10543-1-AP

    WB analysis of mouse spleen using 10543-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 10543-1-AP

    WB analysis of A431 using 10543-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 10543-1-AP

    WB analysis of RAW 264.7 using 10543-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10543-1-AP (PSME1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10543-1-AP

    IHC staining of human lung cancer using 10543-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME1 antibody included
  • IHC staining of human lung cancer using 10543-1-AP

    IHC staining of human lung cancer using 10543-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10543-1-AP (PSME1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSME1 antibody included

Tested Applications

Positive WB detected inmouse spleen tissue, A431 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSME1 Ready-To-Use IHC Kit. PSME1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10543-1-AP targets PSME1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSME1 fusion protein Ag0831 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Calculated molecular weight 29 kDa
Observed molecular weight 29-33 kDa
GenBank accession numberBC007503
Gene symbol PSME1
Gene ID (NCBI) 5720
RRIDAB_2170944
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

Protocols

Product Specific Protocols
WB protocol for PSME1 antibody 10543-1-APDownload protocol
IHC protocol for PSME1 antibody 10543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proteomics

Proteomic analysis of the lungs of mice infected with different pathotypes of H5N1 avian influenza viruses.

Authors - Zhao Dongming D
View Article