CoraLite® Plus 488-conjugated PSMB2 Monoclonal antibody
PSMB2 Monoclonal Antibody for IF, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3C5C5
Cat no : CL488-68180
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | HepG2 cells, PC-3 cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-68180 targets PSMB2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PSMB2 fusion protein Ag7308 相同性解析による交差性が予測される生物種 |
Full Name | proteasome (prosome, macropain) subunit, beta type, 2 |
Calculated molecular weight | 23 kDa |
Observed molecular weight | 23 kDa |
GenBank accession number | BC000268 |
Gene symbol | PSMB2 |
Gene ID (NCBI) | 5690 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 PSMB2 antibody CL488-68180 | Download protocol |
FC protocol for CL Plus 488 PSMB2 antibody CL488-68180 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |