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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using PSMA5 Recombinant antibody (84424-4-RR)

    IHC staining of mouse liver using 84424-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84424-4-RR (PSMA5 antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSMA5 Recombinant antibody (84424-4-RR)

    IHC staining of mouse brain using 84424-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84424-4-RR (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA5 Recombinant antibody (84424-4-RR)

    IF Staining of HeLa using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMA5 Recombinant antibody (84424-4-RR)

    IF Staining of U2OS using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA5 Recombinant antibody (84424-4-RR)

    IF Staining of HeLa using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Flow cytometry (FC) experiment of HepG2 cells using PSMA5 Recombinant antibody (84424-4-RR)

    FC experiment of HepG2 using 84424-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA5 Recombinant antibody (84424-4-RR, Clone:241629H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84424-4-RR

    Affinity and Kinetic Characterization of 84424-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84424-4-RR against Human PSMA5 were performed. The affinity constant is 36.6 pM.

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PSMA5 Recombinant antibody

PSMA5 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84424-4-RR
Clone No.241629H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

241629H2, Proteasome subunit alpha type-5, PSC5, ZETA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U2OS cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84424-4-RR targets PSMA5 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMA5 fusion protein Ag34928 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 5
Calculated molecular weight 241 aa, 26 kDa
GenBank accession numberBC103751
Gene Symbol PSMA5
Gene ID (NCBI) 5686
RRIDAB_3671951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

Protocols

Product Specific Protocols
IHC protocol for PSMA5 antibody 84424-4-RRDownload protocol
IF protocol for PSMA5 antibody 84424-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols