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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA5 Recombinant antibody (84424-4-RR)

    IF Staining of HeLa using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMA5 Recombinant antibody (84424-4-RR)

    IF Staining of U2OS using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84424-4-RR

    Affinity and Kinetic Characterization of 84424-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84424-4-RR against Human PSMA5 were performed. The affinity constant is 36.6 pM.

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PSMA5 Recombinant antibody

PSMA5 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84424-4-RR
Clone No.241629H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

ZETA, PSC5, Proteasome subunit alpha type-5, 241629H2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84424-4-RR targets PSMA5 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMA5 fusion protein Ag34928 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 5
Calculated molecular weight 241 aa, 26 kDa
GenBank accession numberBC103751
Gene Symbol PSMA5
Gene ID (NCBI) 5686
RRIDAB_3671951
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

Protocols

Product Specific Protocols
IF protocol for PSMA5 antibody 84424-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols