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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA5 Recombinant antibody (84424-4-RR)

    IF Staining of HeLa using 84424-4-RR (same clone as 84424-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMA5 Recombinant antibody (84424-4-RR)

    IF Staining of U2OS using 84424-4-RR (same clone as 84424-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01286-3

    Cytometric bead array standard curve of MP01286-3

    Cytometric bead array standard curve of MP01286-3, PSMA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84424-4-PBS. Detection antibody: 84424-1-PBS. Standard: Ag34928. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 84424-4-RR

    Affinity and Kinetic Characterization of 84424-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84424-4-RR against Human PSMA5 were performed. The affinity constant is 36.6 pM.

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PSMA5 Recombinant antibody, PBS Only (Capture)

PSMA5 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84424-4-PBS
Clone No.241629H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

ZETA, PSC5, Proteasome subunit alpha type-5, 241629H2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84424-4-PBS targets PSMA5 as part of a matched antibody pair:

MP01286-3: 84424-4-PBS capture and 84424-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMA5 fusion protein Ag34928 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 5
Calculated molecular weight 241 aa, 26 kDa
GenBank accession numberBC103751
Gene Symbol PSMA5
Gene ID (NCBI) 5686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28066
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)