Validation Data Gallery
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Tested Applications
| Positive WB detected in | C6 cells, A549 cells, NIH/3T3 cells |
| Positive IHC detected in | human kidney tissue, human placenta tissue, human colon tissue, mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:800 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16168-1-AP targets PSEN2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | presenilin 2 (Alzheimer disease 4) |
| Calculated molecular weight | 50 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | NM_000447 |
| Gene Symbol | PSEN2 |
| Gene ID (NCBI) | 5664 |
| RRID | AB_2170748 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49810 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Presenilins can regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. These transmembrane proteins are considered to be involved in early onset of Alzheimer's disease. Presenilin 2 mainly localizes in large neurons. It exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (about 23 kDa) fragments.This antibody is specific to presenilin 2. It will not bind presenilin 1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PSEN2-Specific antibody 16168-1-AP | Download protocol |
| WB protocol for PSEN2-Specific antibody 16168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gut Microbes Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis | ||
Neural Regen Res MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons |
