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  • Featured Product
  • KD/KO Validated

PSEN2-Specific Polyclonal antibody

PSEN2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16168-1-AP

Synonyms

AD3L, AD3LP, AD4, AD5, E5 1, Presenilin 2, PS 2, PS2, PSEN2, PSEN2-Specific, PSNL2, STM 2, STM2

Validation Data Gallery

Applications:
  • WB analysis using 16168-1-AP

    WB analysis using 16168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 16168-1-AP

    WB analysis of A549 using 16168-1-AP

    WB result of PSEN2-Specific antibody (16168-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSEN2-Specific transfected A549 cells.

  • IHC staining of human kidney using 16168-1-AP

    IHC staining of human kidney using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 16168-1-AP

    IHC staining of human kidney using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human placenta using 16168-1-AP

    IHC staining of human placenta using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human placenta using 16168-1-AP

    IHC staining of human placenta using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of mouse brain using 16168-1-AP

    IHC staining of mouse brain using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 16168-1-AP

    IHC staining of human colon using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 16168-1-AP

    IHC staining of human colon using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16168-1-AP (PSEN2-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 16168-1-AP

    IHC staining of human breast cancer using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 16168-1-AP

    IHC staining of human breast cancer using 16168-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16168-1-AP (PSEN2-Specific antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inC6 cells, A549 cells, NIH/3T3 cells
Positive IHC detected inhuman kidney tissue, human placenta tissue, human colon tissue, mouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:800
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16168-1-AP targets PSEN2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name presenilin 2 (Alzheimer disease 4)
Calculated molecular weight 50 kDa
Observed molecular weight55-60 kDa
GenBank accession numberNM_000447
Gene symbol PSEN2
Gene ID (NCBI) 5664
RRIDAB_2170748
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Presenilins can regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. These transmembrane proteins are considered to be involved in early onset of Alzheimer's disease. Presenilin 2 mainly localizes in large neurons. It exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (about 23 kDa) fragments.This antibody is specific to presenilin 2. It will not bind presenilin 1.

Protocols

Product Specific Protocols
WB protocol for PSEN2-Specific antibody 16168-1-APDownload protocol
IHC protocol for PSEN2-Specific antibody 16168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Gut Microbes

Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis

Authors - Jinghua Jin
View Article