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Validation Data Gallery

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  • Western Blot (WB) analysis of human milk using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of human milk using 66473-1-Ig (same clone as 66473-1-PBS)

    human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HepG2 using 66473-1-Ig (same clone as 66473-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of PC-3 using 66473-1-Ig (same clone as 66473-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PSAP Monoclonal antibody, PBS Only

PSAP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66473-1-PBS
Clone No.2F11B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator, A1 activator

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66473-1-PBS targets PSAP in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1194

Product name: Recombinant human PSAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC001503

Sequence: MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEF

相同性解析による交差性が予測される生物種
Full Name prosaposin
Calculated molecular weight 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_2881839
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP07602
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.