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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROSC Recombinant antibody (83831-5-RR)

    WB analysis using 83831-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83831-5-RR (PROSC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PROSC Recombinant antibody (83831-5-RR)

    IHC staining of mouse liver using 83831-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83831-5-RR (PROSC antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83831-5-RR

    Affinity and Kinetic Characterization of 83831-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83831-5-RR against Human PROSC were performed. The affinity constant is 2.00 nM.

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PROSC Recombinant antibody

PROSC Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83831-5-RR
Clone No.240998B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PLPBP, 240998B6, PLP homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, Pyridoxal phosphate homeostasis protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, Jurkat cells, L02 cells, mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:325-1:1300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83831-5-RR targets PROSC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PROSC fusion protein Ag18369 相同性解析による交差性が予測される生物種
Full Name proline synthetase co-transcribed homolog (bacterial)
Calculated molecular weight 275 aa, 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012334
Gene Symbol PROSC
Gene ID (NCBI) 11212
RRIDAB_3671415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO94903
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).

Protocols

Product Specific Protocols
WB protocol for PROSC antibody 83831-5-RRDownload protocol
IHC protocol for PROSC antibody 83831-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols