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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROSC Recombinant antibody (83831-5-RR)

    WB analysis using 83831-5-RR (same clone as 83831-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83831-5-RR (PROSC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83831-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PROSC Recombinant antibody (83831-5-RR)

    IHC staining of mouse liver using 83831-5-RR (same clone as 83831-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83831-5-RR (PROSC antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83831-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83831-5-RR

    Affinity and Kinetic Characterization of 83831-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83831-5-RR against Human PROSC were performed. The affinity constant is 2.00 nM.

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PROSC Recombinant antibody, PBS Only

PROSC Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83831-5-PBS
Clone No.240998B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PLPBP, 240998B6, PLP homeostasis protein, Proline synthase co-transcribed bacterial homolog protein, Pyridoxal phosphate homeostasis protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83831-5-PBS targets PROSC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PROSC fusion protein Ag18369 相同性解析による交差性が予測される生物種
Full Name proline synthetase co-transcribed homolog (bacterial)
Calculated molecular weight 275 aa, 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012334
Gene Symbol PROSC
Gene ID (NCBI) 11212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO94903
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human Pyridoxal 5′-phosphate-binding protein (PLPBP; previously proline synthase cotranscribed homolog, PROSC) was first described in 1999 and was demonstrated to be ubiquitously expressed in many tissues. PROSC is ubiquitously expressed in human tissues and is highly conserved throughout evolution. PROSC deficiency could therefore markedly increase free cellular PLP levels (PMID: 31825581, 27912044).