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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKRIP1 Polyclonal antibody (16794-1-AP)

    WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRKRIP1 Polyclonal antibody (16794-1-AP)

    WB analysis using 16794-1-AP (same clone as 16794-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IP experiment of NIH/3T3 using 16794-1-AP (same clone as 16794-1-PBS)

    IP result of anti-PRKRIP1 (IP:16794-1-AP, 4ug; Detection:16794-1-AP 1:2000) with NIH/3T3 cells lysate 1200 ug. This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IHC staining of human colon cancer using 16794-1-AP (same clone as 16794-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16794-1-AP (PRKRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IF Staining of Caco-2 using 16794-1-AP (same clone as 16794-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PRKRIP1 antibody (16794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16794-1-PBS in a different storage buffer formulation.

PRKRIP1 Polyclonal antibody, PBS Only

PRKRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16794-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

C114, PRKR interacting protein 1, PRKR-interacting protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16794-1-PBS targets PRKRIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10266

Product name: Recombinant human PRKRIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC014298

Sequence: MASPAASSVRPPRPKKEPQTLVIPKNAAEEQKLKLERLMKNPDKAVPIPEKMSEWAPRPPPEFVRDVMGSSAGAGSGEFHVYRHLRRREYQRQDYMDAMAEKQKLYAEFQKRLEKNKIAAEEQTAKRRKKRQKLKEKKLLAKKMKLEQKKQEGPGQPKEQGSSSSAEASGTEEEEEVPSFTMGR

相同性解析による交差性が予測される生物種
Full Name PRKR interacting protein 1 (IL11 inducible)
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight21-25 kDa
GenBank accession numberBC014298
Gene Symbol PRKRIP1
Gene ID (NCBI) 79706
RRIDAB_3741894
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H875
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PRKR-interacting protein 1 (PRKRIP1), a component of the splicing C complex, is a double-stranded RNA-binding protein. PRKRIP1 consists of a fully extended N-terminal loop (residues 51-75) and an 18-turn α helix (residues 76-142) that spans 100 Å (PMID: 28502770). The full-length cDNA contains an open reading frame of 561 bp, which encodes an 184-amino acid polypeptide with a predicted molecular mass of 21 kDa (PMID: 12679338).

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