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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKRIP1 Polyclonal antibody (16794-1-AP)

    WB analysis using 16794-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16794-1-AP (PRKRIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IP experiment of NIH/3T3 using 16794-1-AP

    IP result of anti-PRKRIP1 (IP:16794-1-AP, 4ug; Detection:16794-1-AP 1:2000) with NIH/3T3 cells lysate 1200 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IHC staining of human colon cancer using 16794-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16794-1-AP (PRKRIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using PRKRIP1 Polyclonal antibody (16794-1-AP)

    IF Staining of Caco-2 using 16794-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PRKRIP1 antibody (16794-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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PRKRIP1 Polyclonal antibody

PRKRIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16794-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C114, PRKR interacting protein 1, PRKR-interacting protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16794-1-AP targets PRKRIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKRIP1 fusion protein Ag10266 相同性解析による交差性が予測される生物種
Full Name PRKR interacting protein 1 (IL11 inducible)
Calculated molecular weight 184 aa, 21 kDa
Observed molecular weight21-25 kDa
GenBank accession numberBC014298
Gene Symbol PRKRIP1
Gene ID (NCBI) 79706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H875
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKR-interacting protein 1 (PRKRIP1), a component of the splicing C complex, is a double-stranded RNA-binding protein. PRKRIP1 consists of a fully extended N-terminal loop (residues 51-75) and an 18-turn α helix (residues 76-142) that spans 100 Å (PMID: 28502770). The full-length cDNA contains an open reading frame of 561 bp, which encodes an 184-amino acid polypeptide with a predicted molecular mass of 21 kDa (PMID: 12679338).

Protocols

Product Specific Protocols
WB protocol for PRKRIP1 antibody 16794-1-APDownload protocol
IHC protocol for PRKRIP1 antibody 16794-1-APDownload protocol
IF protocol for PRKRIP1 antibody 16794-1-APDownload protocol
IP protocol for PRKRIP1 antibody 16794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols