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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PRKACB Polyclonal antibody

PRKACB Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12232

Synonyms

DKFZp781I2452, PKA C beta, PKACB, PRKACB

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-12232

    IF Staining of HeLa using CL488-12232

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PRKACB antibody (CL488-12232) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12232-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-12232 targets PRKACB in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKACB fusion protein Ag2870 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, catalytic, beta
Calculated molecular weight 41 kDa, 46 kDa
Observed molecular weight 36-55 kDa
GenBank accession numberBC016285
Gene symbol PRKACB
Gene ID (NCBI) 5567
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PRKACB antibody CL488-12232Download protocol
Standard Protocols
Click here to view our Standard Protocols