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Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using PREP Polyclonal antibody (11536-1-AP)

    WB analysis of human lung using 11536-1-AP (same clone as 11536-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using PREP Polyclonal antibody (11536-1-AP)

    WB analysis of Jurkat using 11536-1-AP (same clone as 11536-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using PREP Polyclonal antibody (11536-1-AP)

    WB analysis of mouse lung using 11536-1-AP (same clone as 11536-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PREP Polyclonal antibody (11536-1-AP)

    IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PREP Polyclonal antibody (11536-1-AP)

    IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PREP Polyclonal antibody (11536-1-AP)

    IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PREP Polyclonal antibody (11536-1-AP)

    IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PREP Polyclonal antibody (11536-1-AP)

    IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PREP Polyclonal antibody (11536-1-AP)

    IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PREP Polyclonal antibody (11536-1-AP)

    IHC staining of human gliomas using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11536-1-AP (PREP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PREP Polyclonal antibody (11536-1-AP)

    IF Staining of A549 using 11536-1-AP (same clone as 11536-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 11536-1-AP (PREP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PREP Polyclonal antibody, PBS Only

PREP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11536-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Post-proline cleaving enzyme, PEP, EC:3.4.21.26

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11536-1-PBS targets PREP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2101

Product name: Recombinant human PREP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 360-710 aa of BC030636

Sequence: ILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP

相同性解析による交差性が予測される生物種
Full Name prolyl endopeptidase
Calculated molecular weight 710 aa, 81 kDa
Observed molecular weight 81 kDa
GenBank accession numberBC030636
Gene Symbol PREP
Gene ID (NCBI) 5550
RRIDAB_2284249
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP48147
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)

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