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KD/KO Validated

PREP Polyclonal antibody

Name: PREP Polyclonal antibody
Brand: Proteintech
SKU: 11536-1-AP

PREP Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11536-1-AP

Synonyms

PE, PEP, Post proline cleaving enzyme, PREP, prolyl endopeptidase

Validation Data Gallery

Applications:

AllWBIHCIF

WB analysis of human lung using 11536-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11536-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 11536-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 11536-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 11536-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11536-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11536-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11536-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 11536-1-AP (PREP antibody) at dilution of 1:50 (under 10x lens).
IF Staining of A549 using 11536-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 11536-1-AP (PREP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 11536-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human lung tissue, Jurkat cells, mouse lung tissue
Positive IHC detected in	human ovary tumor tissue, human colon cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 4 publications below
IF	See 3 publications below

Product Information

11536-1-AP targets PREP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PREP fusion protein Ag2101 相同性解析による交差性が予測される生物種
Full Name	prolyl endopeptidase
Calculated molecular weight	710 aa, 81 kDa
Observed molecular weight	81 kDa
GenBank accession number	BC030636
Gene symbol	PREP
Gene ID (NCBI)	5550
RRID	AB_2284249
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)

Protocols

Product Specific Protocols
WB protocol for PREP antibody 11536-1-AP	Download protocol
IHC protocol for PREP antibody 11536-1-AP	Download protocol
IF protocol for PREP antibody 11536-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Gut Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease. Authors - Koelink Pim J PJ View Article
human	WB,IHC,IF	Oxid Med Cell Longev Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration. Authors - Huo-Liang Zheng KD Validated View Article
human	WB	Int J Biol Macromol A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin. Authors - Hui Zeng View Article
human	WB,IHC,IF	PLoS One Cigarette smoke-induced collagen destruction; key to chronic neutrophilic airway inflammation? Authors - Overbeek Saskia A SA View Article
mouse	IHC	Am J Physiol Lung Cell Mol Physiol Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown. Authors - Braber Saskia S View Article
	IF	J Gen Virol Phosphorylation of the Autographa californica multiple nucleopolyhedrovirus polyhedron envelope protein plays an important role in the formation of the occlusion body envelope. Authors - Jia Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PREP Polyclonal antibody

PREP Polyclonal Antibody for IF, IHC, WB, ELISA

Validation Data Gallery

WB analysis of human lung using 11536-1-AP

WB analysis of Jurkat using 11536-1-AP

WB analysis of mouse lung using 11536-1-AP

IHC staining of human ovary tumor using 11536-1-AP

IHC staining of human ovary tumor using 11536-1-AP

IHC staining of human colon cancer using 11536-1-AP

IHC staining of human colon cancer using 11536-1-AP

IHC staining of human gliomas using 11536-1-AP

IF Staining of A549 using 11536-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease.

Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration.

A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Cigarette smoke-induced collagen destruction; key to chronic neutrophilic airway inflammation?

Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown.

Phosphorylation of the Autographa californica multiple nucleopolyhedrovirus polyhedron envelope protein plays an important role in the formation of the occlusion body envelope.