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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated PRC1 Monoclonal antibody

PRC1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E1D5

Cat no : CL594-67027

Synonyms

ASE1, PRC1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-67027

    IF Staining of HeLa using CL594-67027

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PRC1 antibody (CL594-67027, Clone: 1E1D5 ) at dilution of 1:100.

  • IF Staining of HeLa using CL594-67027

    IF Staining of HeLa using CL594-67027

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PRC1 antibody (CL594-67027, Clone: 1E1D5 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67027-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67027 targets PRC1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRC1 fusion protein Ag5907 相同性解析による交差性が予測される生物種
Full Name protein regulator of cytokinesis 1
Calculated molecular weight 72 kDa
Observed molecular weight 66-72 kDa
GenBank accession numberBC003138
Gene symbol PRC1
Gene ID (NCBI) 9055
RRIDAB_2920075
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

Protocols

Product Specific Protocols
IF protocol for CL594 PRC1 antibody CL594-67027Download protocol
Standard Protocols
Click here to view our Standard Protocols