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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis using 13482-1-AP (same clone as 13482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of HEK-293T using 13482-1-AP (same clone as 13482-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    WB analysis of human liver using 13482-1-AP (same clone as 13482-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using PPP2CA Polyclonal antibody (13482-1-AP)

    IP experiment of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

    IP result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    IHC staining of human liver using 13482-1-AP (same clone as 13482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PPP2CA Polyclonal antibody (13482-1-AP)

    IHC staining of human liver using 13482-1-AP (same clone as 13482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    IF Staining of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PPP2CA Polyclonal antibody (13482-1-AP)

    FC experiment of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13482-1-PBS targets PPP2CA in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2CA fusion protein Ag4294 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Calculated molecular weight 309 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC031696
Gene Symbol PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2169485
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP67775
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.