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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPIG Polyclonal antibody (12985-1-AP)

    WB analysis using 12985-1-AP (same clone as 12985-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PPIG Polyclonal antibody (12985-1-AP)

    IHC staining of human prostate cancer using 12985-1-AP (same clone as 12985-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12985-1-AP (PPIG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIG Polyclonal antibody (12985-1-AP)

    IF Staining of HeLa using 12985-1-AP (same clone as 12985-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12985-1-AP (PPIG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIG Polyclonal antibody (12985-1-AP)

    IF Staining of HeLa using 12985-1-AP (same clone as 12985-1-PBS)

    Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PPIG Polyclonal antibody (12985-1-AP)

    IF Staining of HeLa using 12985-1-AP (same clone as 12985-1-PBS)

    Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12985-1-PBS in a different storage buffer formulation.

PPIG Polyclonal antibody, PBS Only

PPIG Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12985-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CARS Cyp, CARS-cyclophilin, CARS-Cyp, CASP10, Clk-associating RS-cyclophilin

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12985-1-PBS targets PPIG in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3622

Product name: Recombinant human PPIG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-355 aa of BC001555

Sequence: MGIKVQRPRCFFDIAINNQPAGRVVFELFSDVCPKTCENFRCLCTGEKGTGKSTQKPLHYKSCLFHRVVKDFMVQGGDFSEGNGRGGESIYGGFFEDESFAVKHNKEFLLSMANRGKDTNGSQFFITTKPTPHLDGHHVVFGQVISGQEVVREIENQKTDAASKPFAEVRILSCGELIPKSKVKKEEKKRHKSSSSSSSSSSDSDSSSDSQSSSDSSDSESATEEKSKKRKKKHRKNSRKHKKEKKKRKKSKKSASSESEAENLEAQPQSTVRPEEIPPIPENRFLMRKSPPKADEKERKNRERERERECNPPNSQPASYQRRLLVTRSGRKIKGRGPRVGDSFPRDLHNIAFVF

相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase G (cyclophilin G)
Calculated molecular weight 739 aa, 87 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC001555
Gene Symbol PPIG
Gene ID (NCBI) 9360
RRIDAB_2169281
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13427
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Peptidyl-prolyl cis-trans isomerase G (commonly abbreviated as PPIG) belongs to the cyclophilin subfamily of peptidyl-prolyl cis-trans isomerases (PPIases), which act as molecular chaperones to accelerate protein folding and regulate vital cellular processes.

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