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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPIG Polyclonal antibody

PPIG Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12985-1-AP

Synonyms

CARS cyclophilin, CARS Cyp, CASP10, Clk associating RS cyclophilin, Cyclophilin G, CYP, Peptidyl prolyl isomerase G, PPIase G, PPIG, Rotamase G, SR cyclophilin, SR cyp, SRCyp

Validation Data Gallery

Applications:
  • WB analysis of human liver using 12985-1-AP

    WB analysis of human liver using 12985-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 12985-1-AP

    WB analysis of SH-SY5Y using 12985-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 12985-1-AP

    WB analysis of NIH/3T3 using 12985-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12985-1-AP

    WB analysis of Jurkat using 12985-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12985-1-AP

    WB analysis of Jurkat using 12985-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 12985-1-AP

    WB analysis of SH-SY5Y using 12985-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12985-1-AP

    IHC staining of human prostate cancer using 12985-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12985-1-AP (PPIG antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HeLa using 12985-1-AP

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12985-1-AP (PPIG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 12985-1-AP

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of HeLa using 12985-1-AP

    IF Staining of HeLa using 12985-1-AP

    Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inhuman liver tissue, Jurkat cells, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12985-1-AP targets PPIG in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIG fusion protein Ag3622 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase G (cyclophilin G)
Calculated molecular weight 739 aa, 87 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC001555
Gene symbol PPIG
Gene ID (NCBI) 9360
RRIDAB_2169281
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPIG antibody 12985-1-APDownload protocol
IHC protocol for PPIG antibody 12985-1-APDownload protocol
IF protocol for PPIG antibody 12985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols