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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPIF Polyclonal antibody (18466-1-AP)

    WB analysis using 18466-1-AP (same clone as 18466-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using PPIF Polyclonal antibody (18466-1-AP)

    WB analysis of HepG2 using 18466-1-AP (same clone as 18466-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using PPIF Polyclonal antibody (18466-1-AP)

    WB analysis of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using PPIF Polyclonal antibody (18466-1-AP)

    WB analysis of rat heart using 18466-1-AP (same clone as 18466-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using PPIF Polyclonal antibody (18466-1-AP)

    IP experiment of mouse heart using 18466-1-AP (same clone as 18466-1-PBS)

    IP result of anti-PPIF (IP:18466-1-AP, 3ug; Detection:18466-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (PPIF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using PPIF Polyclonal antibody (18466-1-AP)

    IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PPIF Polyclonal antibody (18466-1-AP)

    IF Staining of MCF-7 using 18466-1-AP (same clone as 18466-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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PPIF Polyclonal antibody, PBS Only

PPIF Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18466-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

PPIF-Specific, Cyclophilin D, Cyclophilin F, Cyp D, CyP-D

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18466-1-PBS targets PPIF in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIF fusion protein Ag13151 相同性解析による交差性が予測される生物種
Full Name peptidylprolyl isomerase F
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC005020
Gene Symbol PPIF
Gene ID (NCBI) 10105
RRIDAB_2169273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30405
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPIF, also named as Cyclophilin D (CypD), CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF.