Various lysates were subjected to SDS PAGE followed by western blot with 12875-1-AP (PPEF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.

WB result of PPEF1 antibody (12875-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPEF1 transfected HeLa cells. This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.