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  • Featured Product
  • KD/KO Validated

PPEF1 Polyclonal antibody

PPEF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12875-1-AP

Synonyms

PP7, PPEF, PPEF 1, PPEF1, PPP7C

Validation Data Gallery

Applications:
  • WB analysis using 12875-1-AP

    WB analysis using 12875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12875-1-AP (PPEF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12875-1-AP

    WB analysis of HeLa using 12875-1-AP

    WB result of PPEF1 antibody (12875-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPEF1 transfected HeLa cells.

  • IHC staining of mouse testis using 12875-1-AP

    IHC staining of mouse testis using 12875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12875-1-AP

    IHC staining of mouse testis using 12875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, PC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

12875-1-AP targets PPEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPEF1 fusion protein Ag3553 相同性解析による交差性が予測される生物種
Full Name protein phosphatase, EF-hand calcium binding domain 1
Calculated molecular weight 653 aa, 76 kDa
Observed molecular weight 69-76 kDa
GenBank accession numberBC036026
Gene symbol PPEF1
Gene ID (NCBI) 5475
RRIDAB_2166375
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPEF1 antibody 12875-1-APDownload protocol
IHC protocol for PPEF1 antibody 12875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols