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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using human POU4F3 Recombinant antibody (82980-1-RR)

    WB analysis using 82980-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82980-1-RR (POU4F3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POU4F3 Recombinant antibody (82980-1-RR)

    IHC staining of mouse brain using 82980-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POU4F3 Recombinant antibody (82980-1-RR)

    IHC staining of mouse brain using 82980-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using POU4F3 Recombinant antibody (82980-1-RR)

    IF Staining of rat brain using 82980-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using POU4F3 Recombinant antibody (82980-1-RR)

    IF Staining of U2OS using 82980-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of U-251 cells using POU4F3 Recombinant monoclonal antibody (82980-1-RR)

    FC experiment of U-251 using 82980-1-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human POU4F3 (82980-1-RR, Clone:230345F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 82980-1-RR

    Affinity and Kinetic Characterization of 82980-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82980-1-RR against Human POU4F3 were performed. The affinity constant is 0.234 nM.

POU4F3 Recombinant monoclonal antibody

POU4F3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 82980-1-RR
Clone No.230345F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

230345F1, Brain 3C, Brain-3C, Brain-specific homeobox/POU domain protein 3C, Brn 3C

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNeuro-2a cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:175-1:700
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82980-1-RR targets POU4F3 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16004

Product name: Recombinant human POU4F3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 99-184 aa of BC104923

Sequence: HPAALTSHPHHAVHQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDP

相同性解析による交差性が予測される生物種
Full Name POU class 4 homeobox 3
Calculated molecular weight 338 aa, 37 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC104923
Gene Symbol POU4F3
Gene ID (NCBI) 5459
RRIDAB_3670729
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15319
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for POU4F3 antibody 82980-1-RRDownload protocol
IHC protocol for POU4F3 antibody 82980-1-RRDownload protocol
WB protocol for POU4F3 antibody 82980-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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