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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using POU4F3 Recombinant monoclonal antibody (82980-1-RR)

    WB analysis using 82980-1-RR (same clone as 82980-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82980-1-RR (POU4F3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POU4F3 Recombinant monoclonal antibody (82980-1-RR)

    IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using POU4F3 Recombinant monoclonal antibody (82980-1-RR)

    IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using POU4F3 Recombinant monoclonal antibody (82980-1-RR)

    IF Staining of rat brain using 82980-1-RR (same clone as 82980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using POU4F3 Recombinant monoclonal antibody (82980-1-RR)

    IF Staining of U2OS using 82980-1-RR (same clone as 82980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using POU4F3 Recombinant monoclonal antibody (82980-1-RR)

    FC experiment of U-251 using 82980-1-RR (same clone as 82980-1-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human POU4F3 (82980-1-RR, Clone:230345F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using POU4F3 Recombinant monoclonal antibody, PBS Only (82980-1-PBS)

    FC experiment of U2OS using 82980-1-PBS

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug POU4F3 Recombinant monoclonal antibody, PBS Only (82980-1-PBS, Clone:230345F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82980-1-RR

    Affinity and Kinetic Characterization of 82980-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82980-1-RR against Human POU4F3 were performed. The affinity constant is 0.234 nM.

POU4F3 Recombinant monoclonal antibody, PBS Only

POU4F3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 82980-1-PBS
Clone No.230345F1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

230345F1, Brain 3C, Brain-3C, Brain-specific homeobox/POU domain protein 3C, Brn 3C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82980-1-PBS targets POU4F3 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag16004

Product name: Recombinant human POU4F3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 99-184 aa of BC104923

Sequence: HPAALTSHPHHAVHQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDP

相同性解析による交差性が予測される生物種
Full Name POU class 4 homeobox 3
Calculated molecular weight 338 aa, 37 kDa
Observed molecular weight 33-40 kDa
GenBank accession numberBC104923
Gene Symbol POU4F3
Gene ID (NCBI) 5459
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15319
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Protocols

Product Specific Protocols
FC protocol for POU4F3 antibody 82980-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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