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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human gliomas tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of human gliomas using 11084-1-AP (same clone as 11084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11084-1-AP (POLR3K antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP (same clone as 11084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP (same clone as 11084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP (same clone as 11084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP (same clone as 11084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR3K Polyclonal antibody (11084-1-AP)

    IF Staining of HepG2 using 11084-1-AP (same clone as 11084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR3K antibody (11084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR3K Polyclonal antibody (11084-1-AP)

    IF Staining of HepG2 using 11084-1-AP (same clone as 11084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11084-1-AP (POLR3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11084-1-PBS in a different storage buffer formulation.

POLR3K Polyclonal antibody, PBS Only

POLR3K Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 11084-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11084-1-PBS targets POLR3K in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1564

Product name: Recombinant human POLR3K protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-108 aa of BC011932

Sequence: MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
Calculated molecular weight 12 kDa
GenBank accession numberBC011932
Gene Symbol POLR3K
Gene ID (NCBI) 51728
RRIDAB_2165725
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Y1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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