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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of human gliomas using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11084-1-AP (POLR3K antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using POLR3K Polyclonal antibody (11084-1-AP)

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR3K Polyclonal antibody (11084-1-AP)

    IF Staining of HepG2 using 11084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR3K antibody (11084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POLR3K Polyclonal antibody (11084-1-AP)

    IF Staining of HepG2 using 11084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11084-1-AP (POLR3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POLR3K Polyclonal antibody

POLR3K Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 11084-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

HsC11p, hRPC11, DNA-directed RNA polymerase III subunit RPC10, DNA-directed RNA polymerase III subunit K, C11 RNP3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse testis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11084-1-AP targets POLR3K in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1564

Product name: Recombinant human POLR3K protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-108 aa of BC011932

Sequence: MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
Calculated molecular weight 12 kDa
GenBank accession numberBC011932
Gene Symbol POLR3K
Gene ID (NCBI) 51728
RRIDAB_2165725
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Y1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for POLR3K antibody 11084-1-APDownload protocol
IF protocol for POLR3K antibody 11084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols