Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
33482-1-PBS targets POLE2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38070 Product name: Recombinant human POLE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 178-527 aa of BC126218 Sequence: IGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSSNLAIPNHFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKLQGF 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (DNA directed), epsilon 2 (p59 subunit) |
| Calculated molecular weight | 527 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC126218 |
| Gene Symbol | POLE2 |
| Gene ID (NCBI) | 5427 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P56282 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
DNA polymerase epsilon subunit 2 (POLE2) is involved in cellular functions such as DNA replication, repair, and cell cycle control, as well as in the array-based proliferative signatures (PMID: 9745046; 12620412). Knockdown of POLE2 could suppress esophageal squamous cell carcinoma development through regulating cell proliferation, apoptosis, and migration (PMID: 32831648). POLE2 can also regulate the development of oral squamous cell carcinoma by inhibiting the PI3K/AKT signaling pathway (PMID: 38079056).