Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-231 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33482-1-AP targets POLE2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38070 Product name: Recombinant human POLE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 178-527 aa of BC126218 Sequence: IGDAIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAFGFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEVLEKLRIMFAGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKTLADIICEYPDIHQSSRFVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLVNKMCRNCVRFPSSNLAIPNHFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVIADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKLQGF 相同性解析による交差性が予測される生物種 |
| Full Name | polymerase (DNA directed), epsilon 2 (p59 subunit) |
| Calculated molecular weight | 527 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC126218 |
| Gene Symbol | POLE2 |
| Gene ID (NCBI) | 5427 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P56282 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNA polymerase epsilon subunit 2 (POLE2) is involved in cellular functions such as DNA replication, repair, and cell cycle control, as well as in the array-based proliferative signatures (PMID: 9745046; 12620412). Knockdown of POLE2 could suppress esophageal squamous cell carcinoma development through regulating cell proliferation, apoptosis, and migration (PMID: 32831648). POLE2 can also regulate the development of oral squamous cell carcinoma by inhibiting the PI3K/AKT signaling pathway (PMID: 38079056).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for POLE2 antibody 33482-1-AP | Download protocol |
| WB protocol for POLE2 antibody 33482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |