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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PNPLA4 Polyclonal antibody (25469-1-AP)

    WB analysis of HeLa using 25469-1-AP (same clone as 25469-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25469-1-AP (PNPLA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PNPLA4 Polyclonal antibody (25469-1-AP)

    IP experiment of HeLa using 25469-1-AP (same clone as 25469-1-PBS)

    IP result of anti-PNPLA4 (IP:25469-1-AP, 4ug; Detection:25469-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PNPLA4 Polyclonal antibody (25469-1-AP)

    IHC staining of human cervical cancer using 25469-1-AP (same clone as 25469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PNPLA4 Polyclonal antibody (25469-1-AP)

    IHC staining of human cervical cancer using 25469-1-AP (same clone as 25469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25469-1-AP (PNPLA4 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25469-1-PBS in a different storage buffer formulation.

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PNPLA4 Polyclonal antibody, PBS Only

PNPLA4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 25469-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25469-1-PBS targets PNPLA4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PNPLA4 fusion protein Ag22091 相同性解析による交差性が予測される生物種
Full Name patatin-like phospholipase domain containing 4
Calculated molecular weight 253 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC020746
Gene Symbol PNPLA4
Gene ID (NCBI) 8228
RRIDAB_2880095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP41247
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PNPLA4(Patatin-like phospholipase domain-containing protein 4) is also named as DXS1283E, GS2. It participate in triacylglycerol hydrolysis and the acyl-CoA independent transacylation of acylglycerols, there by facilitating energy mobilization and storage in adipocytes. This protein has 2 isoforms produced by alternative splicing.